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PolyU Develops Ultrasensitive Portable Detection Sensor

Image Credits: PolyU, Press Release

As the outbreak of the COVID-19 pandemic has continued for over two years, a fast and accurate antibody detection method can help assess the antibody content of the human body, as well as provide reference data for healthcare departments to formulate appropriate vaccination measures. In the long run, the antibody content data can also contribute to epidemiological studies and vaccine development.

In view of this, a research team from the Department of Applied Physics of The Hong Kong Polytechnic University (PolyU) has successfully developed an ultrasensitive and portable COVID-19 antibody detection sensor based on organic electrochemical transistor (OECT) technology. Fast and easy-to-use, the sensor is designed for testing the antibody levels of people who have either received a vaccination or have been infected with the virus.

Currently, the detection of SARS-CoV-2 immunoglobulin G (IgG) is based on enzyme-linked immunosorbent assay (ELISA) testing of serum samples. Such testing requires the use of bulky medical equipment and can only be performed in hospitals or specialised testing laboratories. It takes about two days to acquire the test results and the cost is high. There is an alternative rapid method based on lateral flow immunoassay (LFIA) testing. However, the sensitivity of this method is lower and incapable of showing the actual level of antibody content.

The COVID-19 antibody detection sensor developed by the PolyU research team comes with an array of advantages – it is highly sensitive, portable, rapid, easy to use and low cost. The testing procedure is very simple. First, the sensor must be turned on and connected to the smartphone app via Bluetooth. Then, 10uL of saliva (or serum) sample is dropped on the transistor test area to allow the antibody-antigen reaction. After five minutes, the saliva sample must be extracted and then added to an electrolyte drop on the test area. The button on the smartphone app is pressed to start the test. After about 20 seconds, the app will display the antibody content level of the saliva sample.

The whole detection process takes less than six minutes and the cost per test is only about HK$ 10. The antibody detection sensor can test the concentration of antibodies from lower than 10fM to higher than 100nM. The detection limit satisfies the need for exquisite analytical sensitivity for saliva.

The Principal Investigator of the study, Professor Feng Yan, Professor of the Department of Applied Physics at PolyU, stated that the research team developed the novel technology by applying voltage pulses on the gate of the sliced transistor to accelerate the incubation process resulting in a significant decrease in the overall detection time.

Since the detection process is non-invasive, using only saliva instead of blood, self-sampling is highly possible. Moreover, the operation and result reading is very simple with the use of a smartphone, and hence the sensor is suitable for instant detection and large-scale screening scenarios.

Looking ahead, the research team aims to apply for funding for clinical studies in collaboration with different medical and testing institutions. The research team expects that the novel device can be commercialised as soon as practicable and applied for antibody detection of other viruses, helping to fight diseases and making contributions to epidemiological studies and vaccine development.

The global COVID-19 diagnostics market size was valued at US$97.4 billion in 2021 and is expected to grow at a compound annual growth rate (CAGR) of 7.7% from 2022 to 2030. The market is driven by the rising government initiatives targeted toward the implementation of mass testing.

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